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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A 55-year-old male with a history of poorly controlled diabetes mellitus develops a necrotizing fasciitis of his left lower extremity following a minor abrasion sustained while gardening. Initialcultures of the wound reveal a polymicrobial flora, including Gram-positive cocci in chains and Gram-negative bacilli. Over the next 48 hours, despite broad-spectrum antibiotic therapy, the patient's condition deteriorates, and new bullae with hemorrhagic fluid appear. Gram stain of this fluid now predominantly shows large, Gram-positive bacilli with subterminal spores. Which of the following virulence factors is MOST likely contributing to the rapid tissue destruction observed in this patient?

A) Protein A
B) Exotoxin B (SpeB)
C) Lecithinase (alpha-toxin)
D) Lipopolysaccharide (LPS)

2. A patient with a history of recurrent urinary tract infections caused by Escherichia coli develops a new infection. The urine isolate is resistant to trimethoprim-sulfamethoxazole. The MOST common mechanism of resistance to this antibiotic combination in E. coli involves:

A) Enzymatic inactivation of trimethoprim and sulfamethoxazole.
B) Expression of efflux pumps that specifically target trimethoprim and sulfamethoxazole.
C) Mutations in the genes encoding dihydrofolate reductase and dihydropteroate synthase.
D) Increased production of para-aminobenzoic acid (PABA).

3. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Acquisition of genes encoding aminoglycoside-modifying enzymes.
B) Increased expression of efflux pumps.
C) Alteration of penicillin-binding proteins (PBPs).
D) Production of beta-lactamase enzymes.

4. What is the most appropriate method for sterilizing a heat-labile liquid solution containing essential proteins for use in tissue culture?

A) Autoclaving at 121°C for 15 minutes
B) Exposure to ethylene oxide gas
C) Filtration through a 0.22 µm pore size membrane filter
D) Irradiation with ultraviolet (UV) light

5. Which pattern on a Kirby-Bauer disk diffusion plate, showing resistance of Staphylococcus aureus to erythromycin but susceptibility to clindamycin with a flattening of the clindamycin zone adjacent to the erythromycin disk (D-zone test positive), indicates inducible clindamycin resistance?

A) Indicates the strain possesses the msrA gene
B) Indicates the strain possesses an erm gene and should be reported as clindamycin resistant
C) Indicates the strain is tolerant to clindamycin
D) Indicates the strain should be reported as clindamycin susceptible

Solutions: